Fig. 3
From: Regulation of mitochondrial iron homeostasis by sideroflexin 2
Mitochondrial targeting signal in SFXN2. The N-terminus (a) or C-terminus (b) of SFXN2 was conjugated to mCherry and then expressed in HeLa cells. SFXN2Nterm-mCherry and mCherry-SFXN2Cterm were diffusely distributed. c SFXN2 was truncated at the end of the second transmembrane domain and conjugated to mCherry. The fusion protein colocalized with MitoTracker. d A SFXN2 fragment containing the N-terminus and the first transmembrane domain was conjugated to mCherry. The fusion protein colocalized with mitochondria. Bars = 5 μm. e Pearson’s colocalization coefficient was calculated to examine the extent of colocalization between the fluorescence of MitoTracker and the fluorescence of each chimeric protein (a–d). n = 5 for SFXN2Nterm-mCherry (Nterm) and mCherry-SFXN2Cterm (Cterm), n = 16 for SFXN2N-TM2-mCherry (TM2), and n = 14 for SFXN2N-TM1-mCherry (TM1). *p < 0.05, ****p < 0.0001 by a one-way ANOVA